Evaluation of silibinin as an efflux pump inhibitor in Bacillus subtilis

Antibiotic resistance has become a global health problem for humankind. Improper use of antibiotics resulted in the increasing evolved bacterial to them. There are different types mechanisms including efflux pumps. To overcome pump activity on drugs, combinatorial therapy existing antimicrobials with natural products is promising insight prevent multidrug resistance. In this study, inhibitory action plant-derived molecule silibinin pumps Bacillus subtilis was investigated. The cellular effect investigated using minimum concentration and growth studies. addition, monitored by ethidium bromide accumulation assay organism. According results, MIC value between 100-200 µgmL-1 microplate 100 inhibited cell growth. Ethidium assays were performed at safe range (25 50 µgmL-1) eliminating death, increased concentration. results proved that significant inhibitor cells candidate eliminate mechanism.